INVESTIGADORES
PITOSSI Fernando Juan
artículos
Título:
Model based analysis of real-time PCR data from DNA binding dye protocols
Autor/es:
ALVAREZ MJ; VILA-ORTIZ GJ; SALIBE MC; PODHAJCER OL; PITOSSI FJ
Revista:
BMC BIOINFORMATICS
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2007 vol. 8 p. 85 - 95
ISSN:
1471-2105
Resumen:
Reverse transcription followed by real-time PCR is widely used for quantification of
specific mRNA, and with the use of double-stranded DNA binding dyes it is becoming a standardspecific mRNA, and with the use of double-stranded DNA binding dyes it is becoming a standard
for microarray data validation. Despite the kinetic information generated by real-time PCR, most
popular analysis methods assume constant amplification efficiency among samples, introducing
strong biases when amplification efficiencies are not the same.
Results: We present here a new mathematical model based on the classic exponential description
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilico
We present here a new mathematical model based on the classic exponential description
of the PCR, but modeling amplification efficiency as a sigmoidal function of the product yield. The
model was validated with experimental results and used for the development of a new method for
real-time PCR data analysis. This model based method for real-time PCR data analysis showed the
best accuracy and precision compared with previous methods when used for quantification of insilicoinsilico
generated and experimental real-time PCR results. Moreover, the method is suitable for the
analyses of samples with similar or dissimilar amplification efficiency.
Conclusion: The presented method showed the best accuracy and precision. Moreover, it does
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
not depend on calibration curves, making it ideal for fully automated high-throughput applications.
The presented method showed the best accuracy and precision. Moreover, it does
not depend on calibration curves, making it ideal for fully automated high-throughput applications.