Glycogen brain branching enzyme

TOLMASKY DS, LABRIOLA C, KRISMAN CR

Cellular and molecular biology (Noisy-le-Grand, France)

C.M.B. Association

Lugar: France; Año: 1998 vol. 44 p. 455 - 460

1165-158X

Rat brain glycogen branching enzyme was partially purified in order to elucidate its mechanism of action. The alpha1,4-alpha1,6-glucan polysaccharide was synthesized using rat brain branching enzyme under two different elongation conditions: Glc-1-P and phosphorylase or UDP-Glc and glycogen synthase. The products obtained demonstrated that the cpolysaccharides synthesized (pattern of the spectra obtained in the presence of Krisman´s reagent, lambda max, parameter A and R, % beta-amylolysis and degree of branching) under different incubation times are nearly constant. These results imply that the degree of branching of a polysaccharide depends only on the enzyme specificity.