Effects of Solvents and Surfactants on the Mycelial Growth of Fusarium oxysporum and Botrytis cinerea

MARÍA LUCRECIA ARIAS CASSARÁ; OLGA BAINO; JUAN CARLOS RAMALLO; MARÍA INÉS YBARRA

BIOCELL

Inca

Lugar: Mendoza; Año: 2006 vol. 30 p. 202 - 202

0327-9545

Synthetic fungicides represent a significant risk to human health. Thus, there is an urgent need for alternative approaches, as natural products, able to control phytopathogenic fungi. Currently, we are evaluating the amounts of solvents and surface polymers to be used to dissolve the treatments, and to improve their dispersion in aqueous media, respectively. The concentrations of solvents and surfactants should not be inhibitory to the fungus strain. We assessed ethanol, ethylacetate and DMSO at 2Ð4% with and without PEG 400 at 2%. Fungicidal activity of solvent and surfactant were evalu- ated using an agar dilution assay. The centre of each PGA plate (control and treated) was inoculated with a plug of growing mycelia and was later incubated until the mycelia reaches the Petri dish border. Mycelial growth diameters were measured for control and treated experiments (3 replicates) and results were analyzed statis- tically. Fusarium oxysporum was not inhibited by treatments with EtOH (2%), EtOH (2%)- PEG, EtOAc (2%), EtOAc (4%)-PEG, and DMSO (2%)-PEG, while Botrytis cinerea was not inhibited by EtOAc (2%) and EtOAc (2%)-PEG.