INVESTIGADORES
VIZIOLI Nora Matilde
artículos
Título:
Study of peptide-ligand interactions in open-tubular capillary columns covalently modified with porphyrins
Autor/es:
YONE A; CARBALLO R; GRELA D; REZZANO IN; VIZIOLI NM
Revista:
ELECTROPHORESIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2011 vol. 32 p. 2840 - 2847
ISSN:
0173-0835
Resumen:
The inner surface of fused silica capillaries has been covalently modified with different
porphyrins (deuteroporphyrin, complexes of deuteroporphyrin with metal ions Fe(III),
Cu(II), Zn(II), Ni(II), and Cu(II)mesotetra (carboxyphenyl) porphyrin) and it was
applied for the separation of biologically active peptides by open-tubular capillary electrochromatography.
Separations were performed in a mobile phase composed of 25mM
potassium phosphate, pH 4.0, 5%v/v ACN and 10mM hydroquinone. Changes in the
effective electrophoretic mobility of peptides were studied concerning porphyrin central
metal atom, attachment geometry, and the presence of coordinating or aromatic amino
acid residues in the peptide sequence. The results showed that differences in metal core
on the porphyrin and the spatial conformation of attached porphyrin result in changes in
the analyte interaction with the stationary phase.