Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease

CHAIN, CECILIA YAMIL; PIRES SOUTO, DÊNIO EMANUEL; SBARAGLINI, MARÍA LAURA; LABRIOLA, CARLOS A.; DAZA MILLONE, MARÍA ANTONIETA; RAMIREZ, EDUARDO ALEJANDRO; CISNEROS, JOSÉ SEBASTIÁN; LOPEZ-ALBIZU, CONSTANZA; SCOLLO, KARENINA; KUBOTA, LAURO T.; RUIZ, ANDRÉS MARIANO; VELA, MARÍA ELENA

ACS Infectious Diseases

Washington, DC: Publicaciones de la AEC, [2015]

Año: 2019 vol. 5 p. 1813 - 1819

2373-8227

ABSTRACT: trans-Sialidase and cruzipain are important virulence factorsfrom Trypanosoma cruzi, the etiological agent of Chagas disease, that havehighly antigenic domains in their structure and were reported as potentialtools for diagnosis of the illness. The aim of the present study is to assessthe possibility of using cruzipain and the catalytic domain of trans-sialidasein a Surface Plasmon Resonance-based immunosensor for the diagnosis ofchronic Chagas disease. Immunoassays carried out with canine seraverified that cruzipain allows the detection of anti-Trypanosoma cruziantibodies whereas recombinant trans-sialidase did not yield specificdetections, due to the high dilutions of serum used in the immunoassaysthat hinder the possibility to sense the specific low titer antibodies. The developed cruzipain-based biosensor, whose price perassay is comparable to a commercial enzyme-linked immunosorbent assay (ELISA), was successfully applied for the rapidquantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA.