CEQUINOR 05415
CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
artículos
Título:
Production, physicochemical characterization, and anticancer activity of methotrexate-loaded phytic acid-chitosan nanoparticles on HT-29 human colon adenocarcinoma cells
Autor/es:
ROJAS, JOHN; CARABALI, GUSTAVO A.; DI VIRGILIO, ANA LAURA; SALAMANCA, CONSTAIN H.; CIRO, YHOR; ALHAJJ, MARIA J.
Revista:
CARBOHYDRATE POLYMERS
Editorial:
ELSEVIER SCI LTD
Referencias:
Año: 2020 vol. 243
ISSN:
0144-8617
Resumen:
Methotrexate-loaded phytic acid-chitosan nanoparticles were synthesized by ionic gelationassisted by high-intensity sonication. The nanoparticles were characterized by particle size,polydispersity index, zeta potential (ZP) and encapsulation efficiency. Their physicalstability was evaluated at 4 °C and 40 °C, whereas the in-vitro methotrexate release was assessed at pH 7.4. The data were heuristically fit to first-order, Higuchi, Peppas-Sahlin andKorsmeyer-Peppas models of release kinetics. Anticancer activity was evaluated using the3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HT-29human colon adenocarcinoma cells. Physicochemical analysis showed that thenanoparticles presented positive ZP values, sizes less than
