CONICET | Buscador de Institutos y Recursos Humanos

Drosophila melanogaster gene tan was originally discovered in the early 20th century as a mutant strain lacking the dark pigment pattern of wild-type (wt) flies and, therefore, showing a light yellowish brown color (McEwen, 1918). Flies lacking Tan function also exhibited abnormalities in vision (Benzer, 1967; Inoue et al., 1988; True et al., 2005), and tan males displayed an abnormal courtship behavior (Cook, 1980; Tomkins et al., 1982). tan1 (t1) and tan3 (t3) alleles were found as spontaneous mutations, t3 mutant being apparently lighter than t1 (Brehme, 1941). tan is the structural gene for N-¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappnergene tan was originally discovered in the early 20th century as a mutant strain lacking the dark pigment pattern of wild-type (wt) flies and, therefore, showing a light yellowish brown color (McEwen, 1918). Flies lacking Tan function also exhibited abnormalities in vision (Benzer, 1967; Inoue et al., 1988; True et al., 2005), and tan males displayed an abnormal courtship behavior (Cook, 1980; Tomkins et al., 1982). tan1 (t1) and tan3 (t3) alleles were found as spontaneous mutations, t3 mutant being apparently lighter than t1 (Brehme, 1941). tan is the structural gene for N-¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 1988; True et al., 2005), and tan males displayed an abnormal courtship behavior (Cook, 1980; Tomkins et al., 1982). tan1 (t1) and tan3 (t3) alleles were found as spontaneous mutations, t3 mutant being apparently lighter than t1 (Brehme, 1941). tan is the structural gene for N-¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 1982). tan1 (t1) and tan3 (t3) alleles were found as spontaneous mutations, t3 mutant being apparently lighter than t1 (Brehme, 1941). tan is the structural gene for N-¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappnert3 mutant being apparently lighter than t1 (Brehme, 1941). tan is the structural gene for N-¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappner¦Â-alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 2005). Tan is expressed as a precursor protein of 43.7 kDa. This precursor is cleaved into two subunits of 29.9 and 13.8 kDa that apparently conform together a heterodimeric active protein (Wagner et al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 2007). The enzyme that generates NBAD from DA, the opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or Ebony protein (Wright, 1987; P¨¦rez et al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 1997), which is codified by the gene ebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappnerebony. Since both Tan and Ebony are involved in cuticle tanning, carcinine regulation, and NBAD metabolism in nervous tissue (Wright, 1987; P¨¦rez et al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 1997, 2004; Hovemann et al., 1998; Borycz et al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 2002; True et al., 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization and histamine in the visual metabolism (Borycz et al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappneret al., 2002; P¨¦rez et al., 2010). During the last few years, several publications appeared regarding NBAD-synthase (Wappner et al., 1996a, b; P¨¦rez et al., 1997, 2002, 2004, 2010; Hovemann et al., 1998; Borycz et al., 2002; Wittkopp et al., 2002; Schachter et al., 2007), but very little is known about tan (True et al., 2005; Wagner et al., 2007). Thus, it was important to further characterize the NBAD-hydrolase in D. melanogaster wt and in mutants t1 and t3.., 1996a, b; P¨¦rez et al., 1997, 2002, 2004, 2010; Hovemann et al., 1998; Borycz et al., 2002; Wittkopp et al., 2002; Schachter et al., 2007), but very little is known about tan (True et al., 2005; Wagner et al., 2007). Thus, it was important to further characterize the NBAD-hydrolase in D. melanogaster wt and in mutants t1 and t3.et al., 2002; Schachter et al., 2007), but very little is known about tan (True et al., 2005; Wagner et al., 2007). Thus, it was important to further characterize the NBAD-hydrolase in D. melanogaster wt and in mutants t1 and t3.et al., 2007). Thus, it was important to further characterize the NBAD-hydrolase in D. melanogaster wt and in mutants t1 and t3.wt and in mutants t1 and t3.