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artículos
Título:
Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
Autor/es:
RIVERO, REBECA E.; CAPELLA, VIRGINIA; CECILIA LIAUDAT, A.; BOSCH, PABLO; BARBERO, CESAR A.; RODRÍGUEZ, NANCY; RIVAROLA, CLAUDIA R.
Revista:
RSC Advances
Editorial:
ELSEVIER
Referencias:
Lugar: Londres; Año: 2020 vol. 10 p. 5827 - 5837
Resumen:
Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because theycan mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties ofa macroporous soft material based on poly(N-isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at 18 C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanicalproperties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix?cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.