PLATELETS BIOENERGETICS SCREENING REFLECTS THE IMPACT OF BRAIN ABETA PLAQUE ACCUMULATION IN A RAT MODEL OF ALZHEIMER

PRESTIA, FEDERICO ARIEL; DO CARMO, SONIA; MARTINO ADAMI, PAMELA VICTORIA; MORELLI, LAURA; CUELLO, AUGUSTO CLAUDIO; GALEANO, PABLO; CASTAÑO, EDUARDO MIGUEL

NEUROCHEMICAL RESEARCH

SPRINGER/PLENUM PUBLISHERS

Lugar: New York; Año: 2019 vol. 44 p. 1375 - 1386

0364-3190

Alzheimers disease is associated to depressed brain energy supply and impaired cortical and hippocampal synaptic function. It was previously reported in McGill-R-Thy1-APP transgenic Tg rats that Abeta deposition per se is sufficient to cause abnormalities in glucose metabolism and neuronal connectivity. These data support the utility of this animal model as a platform for the search of novel AD biomarkers based on bioenergetic status. Recently it has been proposed that energy dysfunction can be dynamically tested in platelets of nonhuman primates. PLTs are good candidates to find peripheral biomarkers for AD because they may reflect in periphery the bioenergetics deficits and the inflammatory and oxidative stress processes taking place in AD brain. In the present study, we carried out a PLTs bioenergetics screening in advanced age 12-14 months-old WT and Tg rats. Results indicated that thrombin activated PLTs of Tg rats showed a significantly lower respiratory rate, as compared to that measured in WT animals, when challenged with the same dose of FCCP, an uncoupler of oxidative phosphorylation. In summary, our results provide original evidence that PLTs bioenergetic profiling may reflect brain bioenergetics dysfunction mediated by Abeta plaque accumulation. Further studies on human PLTs from control and AD patients are required to validate the usefulness of PLTs bioenergetics as a novel blood based biomarker for AD.