A distinctive layering pattern of mouse dentate granule cells is generated by developmental and adult neurogenesis

MATHEWS E.A.; MORGENSTERN N.A.,; PIATTI V.C.,; JESSBERGER S.; ZHAO C,; SCHINDER A.F.; GAGE F. H.

JOURNAL OF COMPARATIVE NEUROLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2010

0021-9967

The dentate gyrus (DG) of the hippocampus develops in an outside-in layering pattern.  However, this phenomenon has never been quantified from embryogenesis through adulthood.  Here, we characterize the relative position in the adult mouse granule cell layer of cells dividing in the embryonic, postnatal, and adult DG, using BrdU and retroviral methodologies.  Further, we identify both developmental and adult-born cohorts that contribute to the adult-dividing population.  Our data support an outside-in layering of the DG that continues through adulthood, and suggest a prominent contribution of early-dividing cells to the adult granule cell layer.  We also find that a significant fraction of the adult subgranular dividing population has been proliferating infrequently since early development.