Flurochloridone-baced herbicides induced genotoxicity effects on Rhinella arenarum tadpoles (Anura: Bufonidae).

NIKOLOFF, N.; NATALE G. S.; MARINO D.; SONOLESKI S.; LARRAMENDY M.L.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2014 vol. 100 p. 275 - 281

0147-6513

Abstract: Acute toxicity and genotoxicity of the flurochloridone (FLC)-containing commercial formulation herbicides Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated on Rhinella arenarum (Anura: Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were evaluated as end points for acute toxicity, whereas frequency of micronuclei (MN) and single cell gel electrophoresis (SCGE) were employed as end points for genotoxicity. Lethality studies revealed equivalent LC-5096 h values of 2.96 and 2.85 mg/L for Twin Pack Gold® and Rainbow®, respectively. Whereas Twin Pack Gold® was not able to induce DNA damage at chromosomal level, Rainbow® increased the frequency of MN only when the lowest concentration (0.71 mg/L) was used. However, all concentrations of Twin Pack Gold® and Rainbow® increased the frequencies of primary DNA lesions estimated by alkaline SCGE. This study represents the first evidence of the acute toxic and genotoxic effects exerted by two FLC-based commercial formulations, Twin Pack Gold® and Rainbow®, on tadpoles from a native amphibian species, R. arenarum, under laboratory conditions. Finally, our findings highlight the importance of minimizing the impacts on nontarget living species exposed to agrochemicals.