Involvement of insulin-like growth factors-I and-II and their receptors in medroxyprogesterone acetate-induced growth of mouse mammary adenocarcinomas

ELIZALDE, PATRICIA V.; LANARI, CLAUDIA; MOLINOLO, ALFREDO A.; GUERRA, FABIANA K.; BALAÑÁ, MARIA E.; SIMIAN, MARINA; IRIBARREN, ADOLFO M.; CHARREAU, EDUARDO H.

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 1998 vol. 67 p. 305 - 317

0960-0760

The role of the insulin-like growth factors (IGFs) system was investigated in hormone-dependent (HD) and -independent (HI) in vivo lines of the medroxyprogesterone acetate (MPA)-induced mammary tumor model in Balb/c mice. IGF-II protein and message showed a three- to four-fold increase in HD lines growing in MPA-treated mice, as compared with HD tumors growing in untreated mice. Progression to a hormone-independent phenotype in all these lines was accompanied by a high constitutive expression of IGF-II. Similar IGF-I mRNA levels were detected in HD and HI lines. Both IGE-I and -II messages arose from the malignant epithelial cells, as shown by in situ hybridization studies. A significant decrease in Man-6P/type II IGF-R content was detected in HD tumors growing in MPA-treated mice as compared with HD lines growing in untreated mice. On the other hand, in HI tumors, notwithstanding high IGF-II synthesis, the levels of Man-6P/type II IGF-R remain high. Competitive inhibition and affinity labeling studies showed an almost exclusive binding of IGF-II to Man-6P/type II IGF-R on tumor membranes. The involvement of IGFs in the growth of epithelial primary cultures of the C4-HD line was evaluated. Exogenous IGF-I potentiated MPA stimulatory effect at concentrations of 50-100 ng/ml. Treatment of C4-HD cells with antisense oligodeoxynucleotides (ASODNs) to type I IGF-R and to IGF-II RNA resulted in a dose-dependent inhibition of MPA-mediated cell proliferation. The inhibition caused by IGF-II ASODNs could not be overcome by the addition of IGF-II up to 150 ng/ml. ASODNs to type I IGF-R at 40 μg/ml reduced by 75% the number of type I IGF-R; ASODNs to IGF-II at 1 μM decreased by 83% the levels of IGF-II protein. Our results provide support for the involvement of IGE-I and -II in MPA-induced mammary tumor growth by autocrine pathways.