DEOXYNIVALENOL INDUCES APOPTOSIS IN CELL CULTURES AND THE ANTIBIOTIC FOSFOMYCIN PROTECTS CELLS FROM NUCLEAR CHANGES

PÉREZ D.S., MARTÍNEZ G., RENSETTI D.E., FERNÁNDEZ PAGGI M.B., RICCIO M.B., PÉREZ S.E., TAPIA M.O., SORACI A.L.

Journal of Veterinary Science & Technology

OMICS Publishing Group

Año: 2015 vol. 06

Deoxynivalenol (DON) is a mycotoxin which inhibits protein synthesis and causes cytotoxicity. Fosfomycin (FOS) is a bactericidal broad-spectrum antibiotic used in human and veterinary medicine with some extra antimicrobial properties. We have demonstrated that after 4 hours of incubation, FOS was able to prevent the cytopathic effect of DON on Hep2 cells. To corroborate these results and to determine whether the effect of DON was due to the induction of apoptosis, Hep2 cells were seeded in 6 well-plates and after 24 hours, cell monolayers were treated with DON (2.8 μg/mL) alone or with DON (2.8 μg/mL) and FOS (550 μg/mL). The presence of nuclear morphological changes representative of apoptosis was evaluated by DAPI staining under immunofluorescence microscope, the percentage of apoptotic cells was determined and the results were evaluated by ANOVA/Tukey test. Cell cultures incubated with DON and FOS were similar to control wells showing the absence of cytotoxicity. Apoptotic cells percentage was significantly higher (5.64+0.27) (p