CIVETAN 23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
Bactericidal Activity and Synergy Studies of Peptide AP-CECT7121 Against Multi-resistant Bacteria Isolated from Human and Animal Soft-tissue Infections
Autor/es:
BISTOLETTI, MARIANA; DELPECH, GASTÓN; CECI, MÓNICA; BRUNI, SERGIO SÁNCHEZ; LISSARRAGUE, SABINA; SPARO, MÓNICA
Revista:
Probiotics and Antimicrobial Proteins
Editorial:
Springer New York LLC
Referencias:
Lugar: New York; Año: 2017 vol. 9 p. 355 - 362
ISSN:
1867-1306
Resumen:
AP-CECT7121 is an antimicrobial peptide, produced by Enterococcus faecalis CECT7121, with bactericidal activity against Gram positive bacteria. The aim of this study was to evaluate the bactericidal activity of AP-CECT7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft-tissue infections. During the period 2014-2015, bacterial strains producing human and animal soft-tissue infections were studied. Samples from patients attended at a general hospital and cattle from four dairies in the Province of Buenos Aires (Argentina), were included. 22 methicillin-resistant Staphylococcus aureus (11, human blood samples; 11, cow milk) and 5 vancomycin-resistant Ent. faecium strains isolated from 4 mastitic dairy cows were tested. AP-CECT7121 (12 mg/L) potency was assessed by time-kill curves alone or with sub-inhibitory concentrations of gentamicin. All staphylococcal strains were susceptible to gentamicin; enterococci did not show high level gentamicin resistance. Colony counts were carried out at 0, 2, 4, 8 and 24 h of incubation. AP-CECT7121 showed bactericidal activity against all the enterococcal strains. In addition, AP-CECT7121 had a bactericidal effect on most staphylococci (16/22). Early AP-CECT7121/gentamicin synergy (4-8 h) for all staphylococci was detected. At 24 h, synergy (19/22) and indifference (3/22) were observed. Synergy with gentamicin was detected for staphylococci. AP-CECT7121 constitutes an attractive candidate for its use as a natural therapeutic tool for the treatment of infections produced by multi-resistant Staph. aureus and vancomycin-resistant Ent. faecium isolated from humans and animals.
