A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides

D. RONCAGLIA; A. SCHMIDT; L. IGLESIAS; A. IRIBARREN

BIOTECHNOLOGY LETTERS

SPRINGER

Año: 2001 vol. 23 p. 1439 - 1443

0141-5492

Base-labile 6-chloro-2,3,5-tri-O-acetylpurine riboside (1c) and 2-amino-6-chloro-2,3,5-tri-O-acetylpurine riboside (1d) were fully deacetylated through Candida antarcticaB lipase hydrolysis, affording respectively6-chloropurine riboside (2c) and 2-amino-6-chloro-purine riboside (2d). Quantitative results were found at pH 7and 60◦Cin24hfor1cand72hfor1d. This mild and simple enzymatic technique represents a convenient procedure for the removal of acetyl groups from such base labile halogenated nucleosides.