IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
artículos
Título:
An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
Autor/es:
ALBERT MASCARELL-CREUS; JOAQUIN CAÑIZARES; JOSEP VILARRASA; SANTIAGO MORA-GARCIA; JOSÉ BLANCA; DANIEL GONZALEZ-IBEAS; MONTSERRAT SALADIÉ; CRISTINA ROIG; WIM DELEU; BELÉN PICÓ; NURIA LÓPEZ-BIGAS; MIGUEL A. ARANDA; JORDI GARCIA-MAS; FERNANDO NUEZ; PERE PUIGDOMÈNECH; ANA CAÑO-DELGADO
Revista:
BMC GENOMICS
Editorial:
BioMed Central Ltd
Referencias:
Lugar: Londres, Reino Unido; Año: 2009 vol. 10 p. 467 - 482
ISSN:
1471-2164
Resumen:
Background
Melon (Cucumis melo) is a horticultural species of significant nutritional value that
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
Cucumis melo) is a horticultural species of significant nutritional value that
belongs to the Cucurbitaceae family, of which economic importance is second only to
the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic
diversity has prompted the development of genetic tools in the last decade. However,
the unprecedented existence of a transcriptomic approaches in melon, highlight the
importance of designing new tools for high-throughput analysis of gene expression.
Results
We report the construction of an oligo-based microarray using a total of 17,510
unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly
enriched with genes that are expressed in fruit and during interaction with pathogens.
Hybridizations for three independent experiments allowed the characterization of
global gene expression profiles during fruit ripening, as well as in response to viral
and fungal infections in plant cotyledons and roots, respectively. Microarray
construction, statistical analyses and validation together with functional-enrichment
analysis are presented in this study.
Conclusions
The platform validation and enrichment analyses shown in our study indicate that this
oligo-based microarray is amenable for future genetic and functional genomic studies
of a wide range of experimental conditions in melon.