INVESTIGADORES
TEN HAVE Arjen
artículos
Título:
The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase.
Autor/es:
GONORAZKY MG; RAMIREZ L; ABD-EL HALIEM A; VOSSEN JH; LAMATTINA L; TEN HAVE A; JOOSTEN MHAJ; LAXALT AM
Revista:
JOURNAL OF PLANT PHYSIOLOGY
Editorial:
ELSEVIER GMBH
Referencias:
Año: 2014 vol. 171 p. 959 - 965
ISSN:
0176-1617
Resumen:
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composedof six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We havepreviously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required forPI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that allsix SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces anearly increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The productionof NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanasealso induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 andSlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expressionof the defense-related genes PR1 and HSR203J.