Validation of a liquid chromatography/tandem mass spectrometry method for the detection of aflatoxin B1 residues in broiler liver

ALEJANDRA P. MAGNOLI; MARÍA L. GONZÁLEZ PEREYRA; MARÍA P. MONGE; LILIA R. CAVAGLIERI; STELLA M. CHIACCHIERA

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Año: 2017

0325-7541

Abstract Aflatoxin B1 is a carcinogenic and mutagenic mycotoxin produced mainly byAspergillus flavus and Aspergillus parasiticus. It is the predominant mycotoxin found in rawmaterials used for the manufacture of broiler feeds. The aim of the present study was todevelop a new and optimized method for the detection and quantification of aflatoxin B1 (AFB1)residues in broiler liver using solid phase extraction (SPE) clean-up and liquid chromatographyelectrosprayionization/tandem mass spectrometry (LC---ESI-MS/MS) detection. The method wasvalidated for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification(LOQ). The validation parameters indicated satisfactory linearity (r2 > 0.99), accuracy andprecision (4.57% intra-day RSD; 14.65% inter-day RSD) a very high recovery (99±13%) andhigh sensitivity achieved for AFB1 in animal samples (LOD = 0.017 and LOQ = 0.050 ng/g). Themethod was effective for the detection and quantification of AFB1 residues in broiler liver andcould also be potentially used for detecting AFB1 in other edible animal tissues after natural orexperimental AFB1 exposure with high sensitivity and precision.