PERSONAL DE APOYO
MARTINEZ Maria Del Carmen
artículos
Título:
EXPERIMENTAL PROTOPORPHYRIA: EFFECT OF BILE ACIDS ON LIVER DAMAGE INDUCED BY GRISEOFULVIN
Autor/es:
MARÍA DEL CARMEN MARTINEZ; SILVINA FERNANDA RUSPINI; SUSANA GRACIELA AFONSO; ROBERTOMEISS; ANA MARIA BUZALEH; ALCIRA BATLLE
Revista:
BioMed Research International
Editorial:
Hindawi Publishing Corporation.
Referencias:
Año: 2015 vol. 2015 p. 1 - 10
ISSN:
2314-6133
Resumen:
The effect of bile acids administration to an experimental mice model of Protoporphyria produced by griseofulvin (Gris) was
investigated. The aim was to assess whether porphyrin excretion could be accelerated by bile acids treatment in an attempt to
diminish liver damage induced by Gris. Liver damage markers, heme metabolism, and oxidative stress parameters were analyzed
in mice treated with Gris and deoxycholic (DXA), dehydrocholic (DHA), chenodeoxycholic, or ursodeoxycholic (URSO). The
administration of Gris alone increased the activities of glutathione reductase (GRed), superoxide dismutase (SOD), alkaline
phosphatase (AP), gamma glutamyl transpeptidase (GGT), and glutathione-S-transferase (GST), as well as total porphyrins,
glutathione (GSH), and cytochrome P450 (CYP) levels in liver. Among the bile acids studied, DXA and DHA increased PROTO IX
excretion, DXA also abolished the action of Gris, reducing lipid peroxidation and hepatic GSH and CYP levels, and the activities of
GGT, AP, SOD, and GST returned to control values. However, porphyrin accumulation was not prevented by URSO; instead this
bile acid reduced ALA-S and the antioxidant defense enzymes system activities. In conclusion, we postulate that DXA acid would
be more effective to prevent liver damage induced by Gris.