Development of a FI-HG-ICP-OES solid phase preconcentration system for inorganic selenium speciation in Argentinean beverages

LUIS A. ESCUDERO; PABLO H. PAHECO; JOSE A. GASQUEZ; JOSE A. SALONIA

FOOD CHEMISTRY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2015 vol. 169 p. 73 - 79

0308-8146

A speciation and preconcentration system has been developed to determine inorganic selenium. Selenium was retained by a column filled with polyvinyl chloride (PVC) with lanthanum hydroxide co-precipitation. Speciation was achieved by introducing a preconcentration step prior selective photoreduction. The retention pH was optimized at 10.0. Two multivariate calibrations and a central composite design were employed for optimization. Sample, reagents and acid flow rates` are significant variables affecting the system. Employing HG-ICP-OES as detection, the optimized system reached a detection limit of 0.03 µg/L, and an enhancement factor of 14875 (25 for preconcentration system, 595 for hydride generation). The system was applied to inorganic selenium speciation in several Argentinean beverages that allow estimate their selenium contribution to diet. In order to verify the accuracy of the method, certified reference materials BCR® 414 Plankton & IRMM-804 Rice Flour were analyzed.