INVESTIGADORES
FELDMAN Mariana Laura
artículos
Título:
Purification and characterization of an endopolygalacturonase produced by Sclerotinia sclorotiorum
Autor/es:
OLIVA CR; REGENTE MR; FELDMAN M.L.; DE LA CANAL L
Revista:
BIOLOGIA PLANTARUM
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1999 vol. 42 p. 609 - 614
ISSN:
0006-3134
Resumen:
An endopolygalacturonase (endo-PG), was purified from the culture medium of a local isolate of Sclerotinia sclerotiorum with ammonium sulphate precipitation, cation exchange chromatography and gel filtration. The purified endo-PG had a molecular mass of approximately 18 kDa estimated by gel filtration. The isoelectric point was determined by isoelectric focusing to be approximately 8, suggesting that PG II possesses a net positive charge at physiological pHs. The pH optimum for the enzyme was at pH 4.5. The endo-PG showed essentially the same affinity for pectin and polygalacturonic acid as substrates.
