Long-term monitoring drug resistance by ultra-deep pyrosequencing in a chronic hepatitis B virus (HBV)-infected patient exposed to several unsuccessful therapy schemes

OJEDA D.; SEDE M.; CASSINO L.; WESTERGAARD G; VAZQUEZ M; BENETTI S; FAY F; TANNO H; QUARLERI J

ANTIVIRAL RESEARCH

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 94 p. 184 - 187

0166-3542

The aim of this study was to analyze the spectrum and dynamics of low-prevalent HBV mutations in the reverse transcriptase (rt) and S antigen by ultra-deep pyrosequencing (UDPS). Samples were obtained from a chronically infected patient who was followed throughout a thirteen-year period. This technology enabled simultaneous analysis of 4084 clonally amplified fragments from the patient allowing detecting low prevalent (