INVESTIGADORES
GALLEGO Susana Mabel
artículos
Título:
Effect of heavy metal ions excess on sunflower leaves. Evidence for involvement of oxidative stress
Autor/es:
SM GALLEGO; MP BENAVIDES; ML TOMARO
Revista:
PLANT SCIENCE
Editorial:
Elsevier
Referencias:
Año: 1996 p. 151 - 159
ISSN:
0168-9452
Resumen:
The relationship between heavy metal ion
toxicity and oxidative stress in plant cells was studied. Leaf segments from 14
day old sunflower seedlings were incubated in solutions containing 0.5 mM
Fe(II), Cu(II) or Cd(I1) ions for 12 h in the light. Treatment with metal ions
studied produced a decrease in chlorophyll and GSH contents as well as increases
in lipid peroxidation and lipoxygenase activity. Free radical scavengers, such
as sodium benzoate and mannitol, prevented the decrease in chlorophyll and GSH
content and the lipid peroxidation and lipoxygenase increases. While Fe(II) and
Cd(II) ions caused a decrease in superoxide dismutase activity, Cu(II) ions
raised its level. However. all three metal ions caused decreases in other
antioxidant enzymes (catalase, ascorbate peroxidase, glutathione reductase and dehydroascorbate
reductase). Free radical scavengers protected these enzymes against inactivation.
No effect of these scavengers was observed on superoxide dismutase activity. These
results indicate that excess Fe(II), Cu(II) or Cd(II) ions produce oxidative
damage in plant leaves.