Genetic variability estimated with RAPD-PCR markers in two populations of the nematodo Heterodera glycines Ichinoe, 1952 (Nematoda: Heteroderidae) from Argentina.

LAX, P.; RONDAN DUEÑAS, J.C.; GARDENAL, C.N Y DOUCET, M.

NEMATOLOGY

Koninklijke Brill NV

Lugar: Leiden; Año: 2004 vol. 6 p. 13 - 21

1388-5545

Knowledge of genetic variability within and between phytophagous nematode populations is important for the selection of suitable control strategies. The nematode Heterodera glycines is the most important pathogen of soybean in many producer countries, causing significant economic losses. The levels of variability were analysed in two populations of the nematode from Argentina, using Random Amplified Polymorphic DNA markers in order to evaluate the genetic population structure of this species. DNA was extracted from single females of populations race 1 and 3. Five primers were selected, and fifty consistent fragments were considered for further analysis. Populations studied showed high levels of genetic diversity. Most of the total variation detected in allele frequencies was attributed to variability among individuals of the same population (83.03%) rather than inter-population differences (16.97%). None of the populations demonstrated exclusive bands. However, 68% of fragments showed significant differences in their allele frequencies. This study revealed an important degree of genetic differentiation between both populations, probably as a consequence of limited gene flow between them or because each population was under different management practices at its site of origin. Results herein represent the first analysis of genetic structure in H. glycines populations using RAPD-PCR technique.