Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences, and decreases bug attack

GIACOMETTI R; BARNETO J; BARRIGA LG; SARDOY PM; BALESTRASSE K; ANDRADE AM; PAGANO EA; ALEMANO SG; ZAVALA, J.A.*

PEST MANAGEMENT SCIENCE

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2016 vol. 72 p. 1585 - 1594

1526-498X

BACKGROUND:Southerngreenstinkbugs(NezaraviridulaL.)invadefield-grownsoybeancrops,wheretheyfeedondeveloping seeds and inject phytotoxic saliva, which causes yield reduction. Although leaf responses to herbivory are well studied, no informationisavailableabouttheregulationofdefencesinseeds.RESULTS:Thisstudydemonstratedthatmitogen-activatedproteinkinasesMPK3,MPK4andMPK6areexpressedandactivated in developing seeds of field-grown soybean and regulate a defensive response after stink bug damage. Although 10?20min afterstinkbugfeedingonseedsinducedtheexpressionofMPK3,MPK6andMPK4,onlyMPK6wasphosphorylatedafterdamage. Herbivory induced an early peak of jasmonic acid (JA) accumulation and ethylene (ET) emission after 3h in developing seeds, whereassalicylicacid(SA)wasalsoinducedearly,andatincreasinglevelsupto72hafterdamage.Damagedseedsupregulated defensivegenestypicallymodulatedbyJA/ETorSA,whichinturnreducedtheactivityofdigestiveenzymesinthegutofstink bugs.Inducedseedswerelesspreferredbystinkbugs.CONCLUSION:Thisstudyshowsthatstinkbugdamageinducesseeddefences,whichisperceivedearlybyMPKsthatmayactivate defencemetabolicpathwaysindevelopingseedsoffield-grownsoybean.