Association between polymorphism of the BoLA DRB3.2* and neutralizing anitbody titers against two bovine viruses.

JULIARENA, MARCELA; CERIANI, CAROLINA; SALA, LIVIO; GUTIÉRREZ, SILVINA ELENA; ODEÓN, ANSELMO; POLI, MARIO; ESTEBAN, EDUARDO

BIOCELL

INST HISTOL EMBRIOL-CONICET

Año: 2008 vol. 32 p. 51 - 51

0327-9545

Introduction: bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BHV-1) produce important economic losses. Although controversial results, vaccination with inactivated vaccines is a common practice. High neutralizing titers against BVDV denote resistance to the infection, and high neutralizing titers against BHV-1 could be associated to resistance to primary infection. Objective: to study association between the BoLA DRB3.2* gene and titers of neutralizing antibodies. Material and methods: cattle from 7 dairy herds from different regions of Argentina were analysed. All the cattle belonged to the Holstein breed, and were more than three years old. Neutralizing antibody titers were determined by neutralizing assays and alleles of the BoLA DRB 3.2* gene were determined by PCR-SSOP. Animals with neutralizing titers >1:128 were considered resistant. Association between antibody titers and alleles was estimated by relative risk (RR). Results: DRB3.2* 1701 allele is associated with high titers of antibodies against BVDV and BHV-1. DRB3.2*1401 or 02 could only be associated with high titers of antibodies against BVDV. Discussion: alleles *1701 and *1401 or 02 are associated to resistance. The design of control programs could be aid by genetic control.