INVESTIGADORES
GUTIERREZ Silvina Elena
artículos
Título:
The complete genomic sequence of an in vivo low replicating BLV strain
Autor/es:
DUBE, SYAMALIMA; ABBOTT, LYNN; DUBE, DIPAK; DOLCINI, GUILLERMINA; GUTIÉRREZ, SILVINA ELENA; CERIANI, CAROLINA; JULIARENA, MARCELA; FERRER, JORGE; PERZOVA, RAISA; POIESZ, BERNARD
Revista:
VIROLOGY JOURNAL
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2009 vol. 6 p. 120 - 131
ISSN:
1743-422X
Resumen:
DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after
inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate,
low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified
with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV
ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences
including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina.
Characterization of BLV ARG 41´s deduced proteins and its relationship to other members of the
PTLV/BLV genus of retroviruses are discussed.