Role of interspecies interactions in dual-species biofilms developed in vitro by uropathogens isolated from polymicrobial urinary catheterassociated bacteriuria

MELISA JACOBS AND LUIS IELPI; MELISA JACOBS AND LUIS IELPI; CELESTE MATEYCA; CELESTE MATEYCA; ESTELA M. GALVÁN; ESTELA M. GALVÁN

BIOFOULING

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2016 vol. 32 p. 1067 - 1077

0892-7014

Most catheter-associated urinary tract infections are polymicrobial. Here, uropathogen interactions in dual-species biofilms were studied. The dual-species associations selected based on their prevalence in clinical settings were Klebsiella pneumoniae?Escherichia coli, E. coli?Enterococcus faecalis, K. pneumoniae?E. faecalis, and K. pneumoniae?Proteus mirabilis. All species developed singlespecies biofilms in artificial urine. The ability of K. pneumoniae to form biofilms was not affected by E. coli or E. faecalis co-inoculation, but was impaired by P. mirabilis. Conversely, P. mirabilis established a biofilm when co-inoculated with K. pneumoniae. Additionally, E. coli persistence in biofilms was hampered by K. pneumoniae but not by E. faecalis. Interestingly, E. coli, but not K. pneumoniae,partially inhibited E. faecalis attachment to the surface and retarded biofilm development. Thefindings reveal bacterial interactions between uropathogens in dual-species biofilms ranged fromaffecting initial adhesion to outcompeting one bacterial species, depending on the identity of thepartners involved.