Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis

BIGI M; BLANCO F; FLABIO ARAUJO; ZUMARRAGA M; CATALDI A; M. SORIA; BIGI F

MICROBIOLOGY AND IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2016 vol. 60 p. 552 - 560

0385-5600

Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans or animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and the other members of the MTC evolved. The genome of M. bovis is over 99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1,200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In this study we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutation in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis.