INVESTIGADORES
GRUNBERG Karina Alejandra
artículos
Título:
Molecular genetic discrimination of Buffel grass genotypes and F1 hybrids for breeding purposes using AFLP (Amplified Fragment Length Polymorphism) analyses
Autor/es:
S. GRIFFA; D. DÍAZ; A. RIBOTTA; S. LANZA CASTELLI; N. MUÑOZ; E. LÓPEZ COLOMBA; C. LUNA; K.GRUNBERG; E. BIDERBOST
Revista:
GRASS AND FORAGE SCIENCE (PRINT)
Editorial:
Blackwell Publishing
Referencias:
Lugar: Inglaterra; Año: 2006 p. 454 - 458
ISSN:
0142-5242
Resumen:
Abstract
Buffel grass (Cenchrus ciliaris L.) is an apomictic species
that is widely adapted to arid and semi-arid, tropical
and warm climates. Six apomictic cultivars used as male
parents, one sexual line used as the female parents and
15 putative F1 genotypes were studied using the
amplified fragment length polymorphism (AFLP) technique
for their evaluation and recognition. The NTSYS
programme and Jaccards Index were used to establish
the genetic similarity; and a phenogram was constructed.
A total of 152 bands was recorded. Three primer
combinations were sufficient to identify the genotypes.
The maximum proportion of polymorphisms was 0Æ835,
and 0Æ510 of the polymorphisms consisted in individual
bands that strictly diagnosed unique genotypes. Texas
4464 and Americana cultivars were the most different
from the sexual line, indicating that they might be
the best genotypes for future crossing. Two hybrids
were identified among F1 material to demonstrate the
usefulness of the AFLP technique in characterizing
genotypes and recognizing hybrids in Buffel grass for
breeding purposes.