INVESTIGADORES
GRUNBERG Karina Alejandra
artículos
Título:
Citología de mucosa bucal en diabéticos tipo II. Estudio morfológico y morfométrico con microscopía laser confocal
Autor/es:
ISMAEL, RODRIGUEZ; KARINA GRUNBERG; CARMEN CARDA; MARÍA ELSA GÓMEZ DE FERRARIS
Revista:
Actual. Med
Editorial:
Real Academia de Medicina y Cirugía de Andalucía Oriental
Referencias:
Año: 2009 vol. 94 p. 18 - 23
ISSN:
0365-7965
Resumen:
In this work we analyzed some cytological extensions of the buccal mucosa epithelium corresponding to
type II diabetes patients using light and confocal laser microsocopy (CLSM). In all cases, exfoliative
cytologies were taken from the cheek oral mucosa and tongue lateral side of 14 diabetic patients and 6
control patients without these antecedents. The obtained samples -cytobrush- were stained using
Papanicolau technique. For the morphological analysis, the eosinophilic index (EI) was determined and
CLSM images were taken for morphometry. Variables analyzed here were: cytoplasmic area (AC) and
nuclear area (AN), and the ratio nucleus-cytoplasm (N/C) was calculated. In diabetic patients, EI varied
according to the analyzed area, with a significant cytoplasmatic amphophilia. AN parameters were
significantly higher in diabetic patients than in controls, especially for lingual specimens. For the lingual
region, N/C index was notably lower in diabetic patients as compared to controls. Cytological analysis and
morphologic and morphometric alterations identified in this work reveal some crucial findings for the
differential diagnosis of syalosis of alcoholic and diabetic origin.