Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat

SANCHEZ, A., M. BILINSKI, V. GONZALEZ NICOLINI, M.J. VILLAR AND J.H. TRAMEZZANI

JOURNAL OF NEUROSCIENCE METHODS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1995 p. 13 - 18

0165-0270

Abstract A simple and efficient procedure for the dispersion and culture of magnocellular neurons of the adult rat hypothalamus was developed. The enzymatically and mechanically dispersed cells were highly viable and showed neurite outgrowth after 3 days of culture. The neurons could be maintained for more than 4 weeks without any sign of deterioration. Light-optic immunocytochemistry of the cultured cells revealed the presence of many oxytocin- and vasopressin-immunoreactive neurons. The results indicate that the method may be suitable for many experimental purposes. PMID: 8869479 [PubMed - indexed for MEDLINE]