Nitric oxide synthase increase in hypothalamic magnocellular neurons after salt loading in the rat. An immunohistochemical and in situ hybridization study

VILLAR, M.J., S. CECCATELLI, M. RÖNNQVIST AND T. HÖKFELT

BRAIN RESEARCH

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1994 vol. 644 p. 273 - 281

0006-8993

Abstract Magnocellular hypothalamic neurons of the paraventricular (PVN) and supraoptic (SON) nuclei have been shown to contain a wide variety of messenger molecules in addition to vasopressin and oxytocin, including the nitric oxide (NO)-synthesizing enzyme (NOS). In this paper we have investigated the effects of salt loading on the expression of NOS by means of immunohistochemistry and in-situ hybridization. The results show an increase in the number of NOS-immunoreactive (IR) neurons both in the PVN and the SON after 5 and 14 days of salt loading. Several of these neurons were double labelled with vasopressin antiserum. In situ hybridization showed a marked increase in the number of neurons expressing NOS mRNA and a stronger signal in individual neurons. The present results suggest a role for NO in the magnocellular hypothalamic system after salt loading. PMID: 7519526 [PubMed - indexed for MEDLINE]