2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

RODRIGO PONTIGGIA, OSVALDO PONTIGGIA, MARINA SIMIAN, JAVIER M. MONTSERRAT, JOACHIM W. ENGELS, ADOLFO M. IRIBARREN

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2010 vol. 20 p. 2806 - 2808

0960-894X

A new synthesis of 20-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 20-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 50-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor a protein (ER-a), and transfection experiments in MCF-7 cell line are also presented.