10-23 DNAzyme modifed with (20R)- and (20S)-20-deoxy-20-C-methyluridine in the catalytic core

LAURA ROBALDO, JAVIER MONTSERRAT, ADOLFO IRIBARREN

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2010 vol. 20 p. 4367 - 4370

0960-894X

The catalytic core of a 10-23 DNAzyme was modified using (20R), (20S)-20-deoxy-20-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg2+ concentrations, indicating that certain 20-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 20-C-methyl-20-deoxynucleosides increased the stability.