INVESTIGADORES
MIRIUKA Santiago Gabriel
artículos
Título:
Human developmental chondrogenesis as a basis for engineering chondrocytes from pluripotent stem cells
Autor/es:
LING WU; CAROLINA BLUGUERMANN; LEVON KYUPELYAN; BROOKE LATOUR; STEPHANIE GONZALEZ; SAUMUYA SHAH; ZORAN GALIC; SUNDI GE; YUHYA ZHU; ALI NSAIR; FRANK PETRIGLIANO; SANTIAGO G. MIRIUKA; XINMIN LI; KAREN LYONS; DAVID MCALLISTER; GAY CROOKS; BEN VAN HANDEL; JOHN ADAMS; DENIS EVSEENKO
Revista:
Stem Cell Reports
Editorial:
Elsevier
Referencias:
Año: 2013
ISSN:
2213-6711
Resumen:
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to
generate articular cartilage using adult stem/progenitor cells have been unsuccessful.
We hypothesized that recapitulation of the human developmental chondrogenic
program using pluripotent stem cells (PSCs) may represent a superior approach for
cartilage restoration. Using laser capture microdissection followed by microarray
analysis, we first defined a surface phenotype
(CD166low/negCD146low/negCD73+CD44lowBMPR1B+) distinguishing the earliest
cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development. From 12
weeks only the superficial layer of articular cartilage was enriched in cells with this pre-
chondrocyte/immature chondrocyte phenotype. Isolation of cells with a similar
immunophenotype from differentiating human PSCs revealed a population of
CD166low/negBMPR1B+ putative immature chondrocytes. Functional characterization
confirmed these cells as committed cartilage progenitors. The identification of a
specific molecular signature for the earliest cartilage-committed cells may provide
essential knowledge for the generation of clinically relevant cartilage cells from PSCs.