Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta.

DIESSLER M; VENTUREIRA, MR; HERNANDEZ R; SOBARZO, C; CASAS L; BARBEITO C; CEBRAL E

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2016

0936-6768

The zonary and endotheliochorial dog placenta is the most invasiveplacenta of carnivores, including that of the cat. The importance ofmetalloproteinases (MMPs) in placenta invasiveness has been determined in severalmammals including species with haemochorial, epitheliochorial and endotheliochorialplacentation. Regarding the latter, the expression of MMP enzymes has beenstudied in the cat and the mature canine placenta. The aim of this work was to analyse the expressionand activity of metalloproteinases MMP-2 and -9 in the early dog placenta. Placentae from 18-30 days of pregnancy were collected. Placental tissue from one uterine horn was fixed immediately in 4 % buffered formaldehyde for immunohistochemistry (IHC). The marginal haematoma, the labyrinth, the non-implantative tissue and theendometrium of the implantative zone of the placentae from the contralateralhorn were dissected and immediately frozen in dry ice to be stored at -70°Cfor the analysis of MMP expression (Westernblot, WB) and activity (zymography, Zg). The results confirmedthe differential expression of MMP-2 and -9 inthe cells of labyrinth and maternal endometrial glands, which directly correlatedwith the levels of MMP expression in the same tissues, by WB. Besides theMMP-2 and -9 expression and mainlygelatinolyticactivity (Zg) in marginal haematoma, only activityof MMP-2 was found in labyrinth and implantative endometrium of early dogplacenta. The results are consistent with the findings in other species inwhich the MMP-2 activation precedes the MMP-9 one in early placentation.