PERSONAL DE APOYO
GIRO Mariana
artículos
Título:
An in vivo system involving co-expression of cyanobacterial flavodoxin and ferredoxin?NADP+ reductase confers increased tolerance to oxidative stress in plants
Autor/es:
MARIANA GIRÓ; ROMINA D. CECCOLI; HUGO O. POLI; NÉSTOR CARRILLO; ANABELLA F. LODEYRO
Revista:
FEBS Open Bio
Editorial:
Mary Purton
Referencias:
Lugar: Cambridge; Año: 2011 vol. 1 p. 7 - 13
ISSN:
2211-5463
Resumen:
Oxidative stress in plants causes ferredoxin down-regulation and NADP+ shortage, over-reduction of the photosynthetic electron transport chain, electron leakage to oxygen and generation of reactive oxygen species (ROS). Expression of cyanobacterial flavodoxin in tobacco chloroplasts compensates for ferredoxin decline and restores electron delivery to productive routes, resulting in enhanced stress tolerance. We have designed an in vivo system to optimize flavodoxin reduction and NADP+ regeneration under stress using a version of cyanobacterial ferredoxin?NADP+ reductase without the thylakoid-binding domain. Co-expression of the two soluble flavoproteins in the chloroplast stroma resulted in lines displaying maximal tolerance to redox-cycling oxidants, lower damage and decreased ROS accumulation. The results underscore the importance of chloroplast redox homeostasis in plants exposed to adverse conditions, and provide a tool to improve crop tolerance toward environmental hardships