Effect of pesticides application on peanut (Arachis hypogaea L.) associated phosphate solubilizing soil bacteria

ANZUAY, MARÍA SOLEDAD; FROLA, ORNELLA; JORGE, GUILLERMO ANGELINI; LUDUEÑA, LILIANA; IBAÑEZ, FERNANDO; FABRA, ADRIANA; TAURIAN, TANIA

APPLIED SOIL ECOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 95 p. 31 - 37

0929-1393

In the peanut-growing area of Córdoba, Argentina, herbicides and fungicides utilization is a common practice. This study analyses the effect of pesticides applied at recommended rates on the number and diversity of phosphate solubilizing soil bacteria from this area and the frequency of pqq genes in this bacterial population. Pesticide soil treatment did not affect the abundance of culturable phosphate solubilizing bacteria but increased their genetic diversity and altered the frequency of pqqE and pqqC genes in this bacterial population. The presence of the pqqE and pqqC genes was observed in a high percentage of isolated Gram-negative phosphate solubilizing bacteria. The analysis of the diversity of 16S rDNA and pqqE genes of soil DNA samples indicated a higher diversity in pesticides treated soil samples compared to control soil samples. Results obtained indicated that pesticides application increases diversity of soil bacterial community and therefore phosphate solubilizing soil bacterial population. Analysis of pqq genes frequency and diversity suggested that they would be potential molecular marker of Gram-negative phosphate solubilizing soil bacteria.