INVESTIGADORES
GOMEZ CASATI Diego Fabian
artículos
Título:
Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
Autor/es:
RIUS, S.; CASATI, P.; IGLESIAS, A.; DIEGO FABIAN GOMEZ CASATI
Revista:
PLANT MOLECULAR BIOLOGY
Editorial:
Springer
Referencias:
Año: 2006 vol. 61 p. 945 - 957
ISSN:
0167-4412
Resumen:
Non-phosphorylating glyceraldehyde-
3-phosphate dehydrogenase (NP-GAPDH) is a conserved
cytosolic protein found in higher plants. In
photosynthetic cells, the enzyme is involved in a shuttle
transfer mechanism to export NADPH from the chloroplast
to the cytosol. To investigate the role of this
enzyme in plant tissues, we characterized a mutant
from Arabidopsis thaliana having an insertion at theArabidopsis thaliana having an insertion at the
NP-GAPDH gene locus. The homozygous mutant was
determined to be null respect to NP-GAPDH, as it
exhibited undetectable levels of both transcription ofgene locus. The homozygous mutant was
determined to be null respect to NP-GAPDH, as it
exhibited undetectable levels of both transcription of
NP-GAPDH mRNA, protein expression and enzyme
activity. Transcriptome analysis demonstrated that the
insertion mutant plant shows altered expression of
several enzymes involved in carbohydrate metabolism.
Significantly, cytosolic phosphorylating (NAD-dependent)
glyceraldehyde-3-phosphate dehydrogenase
mRNA levels are induced in the mutant, which correlates
with an increase in enzyme activity. mRNA
levels and enzymatic activity of glucose-6-phosphate
dehydrogenase were also elevated, correlating with an
increase in NADPH concentration. Moreover,
increased ROS levels were measured in the mutant
plants. Down-regulation of several glycolytic and
photosynthetic genes suggests that NP-GAPDH is
important for the efficiency of both metabolic
processes. The results presented demonstrate that
NP-GAPDH has a relevant role in plant growth and
developmentmRNA, protein expression and enzyme
activity. Transcriptome analysis demonstrated that the
insertion mutant plant shows altered expression of
several enzymes involved in carbohydrate metabolism.
Significantly, cytosolic phosphorylating (NAD-dependent)
glyceraldehyde-3-phosphate dehydrogenase
mRNA levels are induced in the mutant, which correlates
with an increase in enzyme activity. mRNA
levels and enzymatic activity of glucose-6-phosphate
dehydrogenase were also elevated, correlating with an
increase in NADPH concentration. Moreover,
increased ROS levels were measured in the mutant
plants. Down-regulation of several glycolytic and
photosynthetic genes suggests that NP-GAPDH is
important for the efficiency of both metabolic
processes. The results presented demonstrate that
NP-GAPDH has a relevant role in plant growth and
development