Human periprostatic adipose tissue: its influence on prostate cancer cells

SACCA PA,; PISTONE CREYDT V,; CHOI H,; MAZZA ON,; FLETCHER SJ,; FERNÁNDEZ VALLONE VB,; SCORTICATI C,; CHASSEING NA,; CALVO JC,

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY : INTERNATIONAL JOURNAL OF EXPERIMENTAL CELLULAR PHYSIOLOGY, BIOCHEMISTRY, AND PHARMACOLOGY.

KARGER

Lugar: Basel; Año: 2012 vol. 30 p. 113 - 122

1015-8987

BACKGROUND/AIMS: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa). However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) was not studied in comparison to PPAT from PCa patients. We investigated the influence of both PPATs-derived factors on the behavior of androgen-dependent or castration-resistant PCa cells. METHODS: We used conditioned media (CMs) from PPATs from patients with clinically primary PCa (TPPAT) or BPH (BPPAT) on LNCaP and PC3 prostate cancer cell lines. We evaluated cell adhesion, proliferation, migration and metalloproteinases expression after exposure of cells to BPPAT or TPPAT-CMs. RESULTS: The number of LNCaP cells attached to components of TPPAT-CMs significantly decreased compared to cells attached to BPPAT-CMs. This study provides the first evidence that PPAT produces and releases pro-MMP-9. Zymograms demonstrated that TPPAT-CM were more effective in increasing pro-MMP-9 activity than BPPAT-CMs in LNCaP. This result suggested that TPPAT-CMs released factors that could induce the invasive capacity of LNCaP cells. PPAT-CMs only affected PC3 attachment showing that these cells were more adherent to the surface covered with PPAT-CMs than LNCaP. CONCLUSIONS: We conclude that in our system TPPAT factors may be involved in the early stages of the disease. (DOI del manuscrito 10.1159/00033905).