One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

GARRELS, WIEBKE; TALLURI, THIRUMALA R.; APFELBAUM, RONJA ; CARRATALÁ, YANET P.; BOSCH, PABLO; PÖTZSCH, KERSTIN ; GRUESO, ESTHER ; IVICS, ZOLTAN; KUES, WILFRIED A.

Scientific Reports

Nature

Año: 2016

Genetically modified cattle are important for developing new biomedical models andfor an improved understanding of the pathophysiology of zoonotic diseases. However,genome editing and genetic engineering based on somatic cell nuclear transfer suffer from alow overall efficiency. Here, we established a highly efficient one-step multiplex genetransfer system into the bovine genome.