INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
Development and Validation of Capillary Electrophoretic Method for Determination of Inorganic Sulfate into Doxorubicin Loaded Liposomes
Autor/es:
FLOR S.,; ESTEVEZ PABLO; TRIPODI V.,; LEGASPI M.; MAMMARELA C.; LUCANGIOLI SILVIA,
Revista:
CURRENT ANALYTICAL CHEMISTRY
Editorial:
BENTHAM SCIENCE PUBL LTD
Referencias:
Año: 2014 vol. 10 p. 241 - 247
ISSN:
1573-4110
Resumen:
Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency is presented. Sulfate was determined using a fused silica capillary (50 cm x 75 µm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water was necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.