Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells

ZUBIRÍA, MARÍA GUILLERMINA; ALZAMENDI, ANA; MORENO, GRISELDA; REY, MARÍA AMANDA; SPINEDI, EDUARDO; GIOVAMBATTISTA, ANDRÉS

Nutrients

Nutrients Editorial

Lugar: Basel; Año: 2016 vol. 8

We have previously addressed that fructose rich diet (FRD) intake for three weeks increasesthe adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue(RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolicparameters, number of adipocyte precursor cells (APCs) and in vitro adipogenic potential fromcontrol (CTR) and FRD adult male rats. Additionally, we have examined the direct fructose effectson the adipogenic capacity of normal APCs. FRD fed rats had increased plasma levels of insulin,triglyceride and leptin, and RPAT mass and adipocyte size. FACS studies showed higher APCsnumber and adipogenic potential in FRD RPAT pads; data is supported by high mRNA levelsof competency markers: PPARg2 and Zfp423. Complementary in vitro experiments indicate thatfructose-exposed normal APCs displayed an overall increased adipogenic capacity. We conclude thatthe RPAT mass expansion observed in eight week-FRD fed rats depends on combined acceleratedadipogenesis and adipocyte hypertrophy, partially due to a direct effect of fructose on APCs.