The oxidative stress and the mitochondrial dysfunction caused by enditoxemia are prevented by alpha-lipoic acid

VANASCO, VIRGINIA; CIMOLAI, MARÍA CECILIA; EVELSON, PABLO; ALVAREZ, SILVIA

FREE RADICAL RESEARCH

Informa

Lugar: Londres, UK; Año: 2008 vol. 42 p. 815 - 823

1071-5762

The aims of this work were to study the mitochondrial function and to evaluate a) the oxidative stress in real time in an acute model of endotoxemia, and b) the effect of a-lipoic acid (LA, 100 mg/kg) as a therapeutic strategy to be considered. In rats treated with lipopolisaccharide (LPS, 10 mg/kg), a 1.4 fold increase was observed in in situ skeletal muscle chemiluminescence. Experimental sepsis increased oxygen consumption in tissue cubes (1 mm3) by 30% for heart and diaphragm and impaired state 3 mitochondrial respiration rate in the three organs (liver, diaphragm, and heart) studied. Only complex I activity in heart and diaphragm, and complex IV activity in diaphragm, were found impaired in this septic model. The production of NO by submitochondrial membranes was found increased by 80% in diaphragm and by 35% in heart of septic rats. The treatment with LA prevented the oxidative stress and mitochondrial dysfunction observed in this model.