Control of Ascosphaera apis and Paenibacillus larvae by the use of essential oil for obtaining beehive products without toxics residues.

BAILAC,P; GENDE,L; GASCON,A; FRITZ,R;PONZI,M AND EGUARAS,M

Molecular Medicinal Chemistry

IDECEFyN

Lugar: Buenos Aires; Año: 2006 vol. 11 p. 1 - 2

1666-888X

In this work in vitro behaviour of essential oils from wild plant species collected in San Luis has been studied, e.g., Tessaria absinthioides, Baccharis coridifolia, Hetheroteca latifolia, Aloysia gratissima, Eupatorium patens, Lippia juneliana, L. integrifolia and L. turbinata against Ascosphaera apis. Also essential oils of culinary aromatic plants were studied, e.g., Syzygium aromaticum (clove), Cinnamomum zeylanicum (cinnamon), Thymus vulgaris (thyme), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) against Paenibacillus larvae subsp. larvae. Oils that produced the highest inhibition halo were those of the genus Lippia 75% inhibition, Tessaria absinthioides and Hetheroteca latifolia 62.5% inhibition. Aloysia gratissima 50% inhibition with regard to the ketoconazol (1 mg/ml) control. Baccharis coridifolia and Eupatorium patens didn´t show any inhibition halo. MIC values were 250-300 ppm for clove oil, 25-50 ppm for cinnamon oil, 150 ppm for thyme, 300 ppm for anise and 250 ppm for fennel essential oil. For chalkbrood, according to experimental data can be inferred that the chemical composition of the essential oils is related with the fungicidal and bactericide power, which showed the highest inhibition percentage were the oils that contained oxygenated terpenes as major constituents, while oils that didn´t inhibit Ascosphaera apis, the occurrence of these terpenes was non significant. For the American foulbrood disease all analysed oils, whose composition has mainly bencenic compounds showed a good antimicrobial activity, cinnamon oil being which showed better effectiveness against strains of Paenibacillus larvae subsp. larvae .