PERI-IMPLANTATIONAL IN VIVO AND IN VITRO EMBRYO-TROPHOBLAST DEVELOPMENT AFTER PERIGESTATIONAL ALCOHOL EXPOSURE IN CD-1 MOUSE.

PEREZ TITO L; BEVILACQUA E; CEBRAL E

DRUG AND CHEMICAL TOXICOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2014 vol. 37 p. 184 - 197

0148-0545

  Long-term pregestational ethanolexposure induced altered fertilization and preimplantation embryogenesis. Weevaluated preimplantational embryo-trophoblast differentiation, growth andinvasiveness after perigestational ethanol 10% ingestion for 15 days precedingand up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1gestation (control females [CF] with water). In TF-D4, expanded and hatchedblastocyst numbers were significantly reduced (p50.05) versus CF-D4. Abnormal embryosand percentage of pyknotic nuclei were increased, and early blastocyst growth(nuclear number/embryo) and mitotic index was reduced (p<0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantlyreduced total embryos andadvanced embryo type 3 number versusCF-D5 (p50.05). During in vitro development,up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed)and 3 percentages (p50.05) versus controls, whereas embryo type 2 percentage increased (p50.05) versus CF-D5. Embryo-trophoblast growth was studied during cultureby morphometry. Embryo size ranges were classified as small, medium and largeembryos. At 48-hour culture, small and medium embryos of TF had significantlyincreased mean area versus CF (p50.05), whereas large embryos had reduced mean area at24-hour culture. Perigestational alcohol exposure up to days 4?5 induced embryodifferentiation retardation, abnormal blastocyst growth and alterations ofembryo-trophoblast growth and expansion during implantation, suggesting impairedregulation of trophoblast invasion and a relation with early pregnancy lossafter mouse perigestational alcohol consumption.