INVESTIGADORES
BOSCH Pablo
artículos
Título:
Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals
Autor/es:
BOSCH, PABLO; FORCATO, DIEGO; ALESSIO, ANA PAULA; FILI, ALEJANDRO; OLMOS NICOTRA, MARÍA FLORENCIA; LIAUDAT, ANA C.; RODRÍGUEZ, NANCY; TALLURI, THIRUMALA R.; KUES, WILFRIED A.
Revista:
CELLULAR AND MOLECULAR LIFE SCIENCES
Editorial:
BIRKHAUSER VERLAG AG
Referencias:
Lugar: BASEL; Año: 2015 vol. 72 p. 1907 - 1929
ISSN:
1420-682X
Resumen:
Abstract Transgenic farm animals are attractive alternative mammalian models to rodents for the study of developmental, genetic, reproductive and disease-related biological questions, as well for the production of recombinant proteins, or the assessment of xenotransplants for human patients. Until recently, the ability to generate transgenic farm animals relied on methods of passive transgenesis. In recent years significant improvements have been made to introduce and apply active techniques of transgenesis and genetic engineering in these species. These new approaches dramatically enhance the ease and speed with which livestock species can be genetically modified, and allow to performing precise genetic modifications. This paper provides a synopsis of enzyme-mediated genetic engineering in livestock species covering the early attempts to employ naturally occurring DNA modifying proteins to recent approaches working with tailored enzymatic systems.