INVESTIGADORES
BOSCH Pablo
artículos
Título:
Time course of follicular development after bovine ovarian tissue transplantation in male non-obese diabetic severe combined immunodeficient mice
Autor/es:
HERNANDEZ-FONSECA, H. J.; BOSCH, P.; MILLER, D. M.; WININGER, J. D.; MASSEY, J. B.; BRACKETT, B. G.
Revista:
FERTILITY AND STERILITY
Editorial:
Elsevier
Referencias:
Año: 2005 vol. 83 p. 1180 - 1187
ISSN:
0015-0282
Resumen:
Objective: The aim of this study, using a bovine model, was to determine the appropriate interval for xenografted adult and newborn ovarian tissue to develop gonadotropin-responsive follicles.
Design: Controlled experiment.
Setting: Academic research laboratory, Department of Physiology and Pharmacology, The University of Georgia, Athens, Georgia, U.S.A.
Animals: Male non-obese diabetic (NOD) severe combined immunodeficient (SCID) mice (n=20) were hosts of bovine ovarian tissue. Two dairy calves and one adult beef cow were donors of ovarian tissue.
Intervention(s): Newborn and adult bovine ovarian cortical pieces were transplanted to the subcutaneous space of intact male NOD SCID mice. Grafts were recovered following euthanasia at intervals post-transplantation.
Main Outcome Measure(s): Microscopic examination of histologic sections to determine proportions of growing follicles.
Result(s): There was an increase (P< 0.05) in the proportion of primary and secondary follicles on day 55 for the cow and, on day 124 after surgery for calf tissue compared to non-grafted, and xenografted ovarian tissues recovered at previous intervals. These observed increases were accompanied by decreases in proportions of primordial follicles. Results suggest a sudden increase in the proportion of primary and secondary follicles due to progressive development of primordial follicles.
Conclusions(s): In the NOD SCID mouse bovine follicles survived xenotransplantation and underwent development. A longer time interval was required for ovarian follicular development in calf tissues, compared to that in adult cow ovarian tissues after xenotransplantation.