INVESTIGADORES
BIGI Fabiana
artículos
Título:
Antigenic characterization of mycobacteria
Autor/es:
A. ALITO; ROMANO M; BIGI F; ZUMARRAGA M; A. CATALDI
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 1999 vol. 68 p. 293 - 299
ISSN:
0378-1135
Resumen:
Tuberculosis-producing mycobacteria have been previously described in marine mammals(Cousins et al., 1990, 1993; Romano et al., 1995; Bernardelli et al., 1996). The strains belonged tothe M. tuberculosis complex (M. tuberculosis, M. bovis, M. microti and M. africanum), but showedgenetic and biochemical differences. The antigenic composition of mycobacteria isolated from wildseals was analyzed by Western blots, using antibodies against some selected antigens. The antigeniccontent was compared with that of M. bovis, M. tuberculosis and M. microti isolates. The lack ofHsp65 protein in supernatants suggested a low degree of cell lysis in the three-week cultures used.SOD, P27 lipoprotein, MPB64 and antigen 85 were observed in all the strains studied. The wild sealstrains, as well as M. tuberculosis, did not produce MPB70 and MPB83. Only very weak bands ofP36 antigen were observed in culture supernatants from wild seal mycobacteria.Summarizing, the antigenic composition of mycobacterial strains from wild seals is differentfrom M. bovis strains.
